This strategy relies on the limited background activity of the modified Cre recombinase (CreER) in the absence of its inducer, the competitive estrogen receptor ligand, tamoxifen
First panel: tamoxifen fed male and female mice stained in the ganglion cell layer and inner nuclear layer of the retina, with female overall staining slightly less as expected due to X-chromosome inactivation Plasmid pMSCV-MerCreMer-hygro from Dr
The advanced Cre technology utilizes a tamoxifen inducible Cre-estrogen receptor (ER) fusion protein (2, 3)
Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye and Brain; Including Seventeen Carrying a New Inducible-First Constitutive-Ready Allele
Our study not only validates the Tfap2b-CreERT2 transgenic line, but also provides a powerful tool for lineage tracing and genetic targeting of Tfap2b -expressing
In the absence of tamoxifen, Cre recombinase is
We have previously reported a plasmid-based, tamoxifen-inducible gene knockdown system in cultured cells using a combined RNAi and Cre-LoxP system
Changes in the expression of tamoxifen-regulated genes could be induced by manipulating LINC00992’s abundance, suggesting that it is at least partially involved in the
Inducible gene expression systems are favored over stable expression systems in a wide variety of
We have previously reported a plasmid-based, tamoxifen-inducible gene knockdown system in cultured cells using a combined RNAi and Cre-LoxP system
2002; 244:305–318
Cre-ERT2 fusion protein
We generated a tamoxifen-inducible Cre transgenic mice using VIPR2-ERT2CreERT2 expression vector consisting of hs1753 regulatory sequence, Hsp68 promoter, ERT2CreERT2 cDNA, and the polyadenylation site from the SV40 early region
This is in part due to the fact that it enables gene control both spatially and temporally
Richard R
By combining the use of liver-specific promoters driving the expression of
For the generation of an inducible tissue-specific knockout mouse at least two basic mouse lines are required: one that expresses the CreER T2 recombinase under the control of a tissue-specific promoter (in the following referred to as “Cre mouse”; for a selection of available lines, see Table 18
Chemically regulated promoters are among the most common inducible promoters
GenBank File: Plasmid sequence and annotations
LTR promoters ; see article for lentiviral vectors for tet-inducible gene or miRNA expression rtTA OnResources: Browse Tetracycline Plasmids Tet-regulated Lentiviral RNAi Vectors Curated Plasmid Collectionspuromycin selection TetR On Wiederschain 41393 pCW57