The Flp-In™ T-REx™ System allows the generation of stable mammalian cell lines exhibiting tetracycline-inducible expression of a gene of interest from a specific
To achieve a robust and persistent inducible expression of a transgene in cells, it has been recommended to establish a stable cell line that constitutively
The tetracycline repressor (tetR)‐regulated system is a widely used tool to specifically control gene expression in mammalian cells
Generation of stable cell lines with tetracycline-inducible (Tet-on) expression of shRNAs or cDNAs using pTER or pT-Rex DEST30 vectors
This method
These cells stably express T7RNAP and TETR integrated into
Inducible gene expression systems are favored over stable expression systems in a wide variety of basic and applied research areas, including functional genomics, gene therapy, tissue engineering, biopharmaceutical protein production and drug discovery
We report the development and isolation of a cell line, termed HepAD38, that replicates human hepatitis B virus (HBV) under conditions that can be regulated with tetracycline
Growth of Tetracycline-Inducible Stable Cell Lines in Suspension Culture The HEK293S cell line and all the derivatives described in this chapter are capable of suspension growth
Tetracycline-inducible HEK293S stable cell lines have been prepared that express high levels (up to 10 mg/liter) of WT opsin and its mutants only in response to the addition of tetracycline and sodium butyrate
The doxycycline inducible overexpression system is a highly flexible and widely used tool for both in vitro and in vivo studies
The Tet-Off and Tet-On systems are based on the Tet repressor protein (TetR) and tet operator (tetO) DNA elements that control the Tn10-encoded tetracycline resistance operon of Escherichia coli
Spent medium was removed and replaced with fresh medium containing different concentrations (0–2
It affords very tight control over expression compared to other inducible systems and is reversible
To identify small molecules that inhibit cccDNA formation, we developed a more efficient cccDNA
Tetracycline-controlled gene expression is based upon
Tet-inducible cell lines after identifying an ideal founder cell line (15)
The tetracycline-inducible cell line producing WT opsin was grown to near confluence in 10-cm-diameter cell culture dishes