To identify cells with tetracycline-inducible expression of shRNAs targeting MST3, clones were grown in the absence (-) or presence (+) of Tetracycline (Tet) for
We chose a tetracycline-inducible/derepressible system based on a modified Cauliflower mosaic virus 35 S promoter (Triple Op), in which three operator
Tetracycline
The generation of a tightly controlled ObLiGaRe doxycycline inducible SpCas9 (ODInCas9) transgene and its use in targeted ObLiGaRe results in functional
They introduced a truncated version of Xist (non-silencing allele) under the control of a tetracycline-inducible system into an autosome in the autoimmune-prone
In recent years, some strategies derived from tetracycline-inducible system alone, as well as the combined use of Tet-based systems and Cre/lox P switching gene expression system, have been newly The Lenti-X Tet-One Inducible Expression System (Puro) is a tetracycline-inducible lentiviral gene expression system that allows you to produce high titers of recombinant, VSV-G pseudotyped lentiviruses for the purpose of establishing a tightly inducible expression system for your gene of interest in a wide variety of dividing and non
Using a fluorescent reporter gene, we show that optimization of the repression level is necessary for efficient induction using anhydrotetracycline in both 2
The Flp-In™ T-REx™ System allows the generation of stable mammalian cell lines exhibiting tetracycline-inducible expression of a gene of interest from a specific genomic location
The Tet-Off systems have a number of advantages, including robust target induction using a relatively benign effector molecule
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The raised concerns are based on the notion that doxycycline can impair mitochondrial function of Since their inception, tetracycline (Tet)-inducible systems have become the method of choice for transgenic research
Tetracycline and its Structural basis of gene regulation by the tetracycline inducible Tet repressor-operator system
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lactis and S
While The tetracycline-inducible (Tet-based) systems have been shown to be an effective tool for examining the contribution of specific gene functions to the induction and progression of neuropathologies (Corti et al
However, during the past decade, a handful of reports have explicitly called for caution when using this system
Kai Schönig, Central Institute of Mental Health, Mannheim, Germany, focuses on the use of Tet technology in transgenic mice
We have developed an improved version using a tandem construct incorporating a constitutive visual marker gene that allows the rapid and straightforward selection of the best