Antibiotics vary in their sensitivitly to heat and/or light – do not add them to media hotter than about 55 o C and store plates/stocks wrapped in foil if a light-sensitive antibiotic like Tetracycline is used
This involves a vector-encoded selectable marker gene, which in most cases, confers resistance to an antibiotic
The tetracycline-inducible gene expression system, which is composed of reverse tetracycline transactivator (rtTA) and a tetracycline response element, is a useful tool in C
Two Different Tetracycline Resistance Mechanisms, Plasmid-Carried tet(L) MWM, molecular weight marker
To get started, you will first need a primer that complements your plasmid sequence
Tetracycline has been a widely used antibiotic because of its low toxicity and broad spectrum of activity
A protocol was designed for plasmid curing using a novel counter-selectable marker, named pylS ZK-pylT, in Escherichia coli
The plasmid, we had to screen many antibiotic-marker-resistant colonies at gradient antibiotic concentrations 13, 14, 33
e <100 bp for tRNA genes)
They are most commonly found as small circular, These plasmids contain a selectable marker, usually an antibiotic resistance gene, which confers on the bacteria an ability to survive and Currently, many antibiotic resistance genes have been found but only a part of them are used to select and maintain plasmids in bacterial strains, primarily genes encoding resistance to ampicillin and kanamycin (Hershfield et al
The differential antibiotic and fluorescence markers on the donor bacterial chromosome and plasmid allowed us to
This plasmid appears to be able to transfer productively to most, if not all Gram-negative bacteria
Two selectable marker sites or antibiotic resistance genes are present on the genome of this plasmid
Also, the introduction of antibiotic resistance genes into biohazardous strains is not recommended [], and
Development of an antibiotic-free plasmid selection system based on glycine auxotrophy for recombinant protein overproduction in
S1), which was modified from the pNIM1 plasmid 16 carrying the CaSAT1 selection marker for nourseothricin resistance and is capable of expressing GFPmut2 generations, their survival rates were similar on ampicillin plates and on tetracycline plates
A plasmid typically contains an antibiotic resistance gene, which allows bacteria to survive in the presence of a specific antibiotic
The mixture was transferred to a 1-mm cuvette and electroporated at 25 µF
Here we report the adoption of a novel selection marker, mfabI (mutant
Map of the pTK-Hyg Vector